Coding

Part:BBa_K392030

Designed by: Teoh Shao Thing, Rie Takino, Kosuke Torigata   Group: iGEM10_Osaka   (2010-10-25)


yeast secretion tag + C. fermi endoglucanase

C. fermi endoglucanase CenA fused with engineered yeast secretion tag for extracellular expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 153
    Illegal NotI site found at 1297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1210
    Illegal BamHI site found at 346
    Illegal XhoI site found at 708
    Illegal XhoI site found at 957
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 488
    Illegal NgoMIV site found at 1413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 392


[edit]
Categories
Parameters
n/ayeast secretion tag + C. fermi endoglucanase